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Old 08-23-2010, 12:00 PM   #1
mnkyboy
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Location: Seattle, WA

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Default The T4 RNA Ligase in Small RNA Illumina Kit

Does anyone know what the comparable NEB product is for the T4 RNA Ligase is in the Illumina Small RNA kits?

I am assuming it is:

T4 RNA Ligase 2 (dsRNA Ligase) M0239S

Thanks.
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Old 08-23-2010, 06:47 PM   #2
ScottC
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Location: Monash University, Melbourne, Australia.

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Default

You might want to take a look at the NEBNext RNA sample prep kits. They have a master mix version, and also a version with all the separate enzymes.

Cheers.
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Old 05-28-2014, 12:57 AM   #3
shyong
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I think the T4 RNA Ligase 2 is for 3' adapter ligation. T4 RNA ligase is for 5' adapter ligation.
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