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Old 08-23-2010, 12:00 PM   #1
Location: Seattle, WA

Join Date: Mar 2009
Posts: 87
Default The T4 RNA Ligase in Small RNA Illumina Kit

Does anyone know what the comparable NEB product is for the T4 RNA Ligase is in the Illumina Small RNA kits?

I am assuming it is:

T4 RNA Ligase 2 (dsRNA Ligase) M0239S

mnkyboy is offline   Reply With Quote
Old 08-23-2010, 06:47 PM   #2
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Location: Monash University, Melbourne, Australia.

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You might want to take a look at the NEBNext RNA sample prep kits. They have a master mix version, and also a version with all the separate enzymes.

ScottC is offline   Reply With Quote
Old 05-28-2014, 12:57 AM   #3
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I think the T4 RNA Ligase 2 is for 3' adapter ligation. T4 RNA ligase is for 5' adapter ligation.
shyong is offline   Reply With Quote

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