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Old 12-10-2010, 08:24 AM   #1
ntremblay
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Location: Montreal, Quebec, Canada

Join Date: Dec 2009
Posts: 30
Default Agencourt Ampure purification kit - exp date

Hi,

does anybody has experienced with an expired ampure kit?

the product datasheet says that the yield is consistent throughout the 12 months shelf life, but I wonder how much it impacts the performance afterward,

thanks for any information,

Nick
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Old 03-05-2012, 08:35 AM   #2
ParkerKB
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Location: Ontario, Canada

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I know this is an old post but just came across it...I haven't tried this yet but a number of other posts suggest titrating your ampure beads using a DNA ladder/marker. See the Roche technical bulletin 2011-002 (attached). That would give you an idea of how functional the beads are after expiring.
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File Type: pdf TCB-11002_GSFLXSystemShortFragmentRemovalProcedure.pdf (318.4 KB, 189 views)
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Old 03-06-2012, 03:51 AM   #3
mboth
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I have just run a test on our AMPure XP beads, between new beads with ones that were 4 months over their expiry date. I compared different titrations on a ladder, and saw no difference whatsoever!
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Old 03-06-2012, 04:41 AM   #4
TonyBrooks
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It's hit and miss for us.
Sometimes they work well, sometimes we've run samples with out of date beads and lost everything. I guess some of it may depend on how many times they've been used (i.e. brought up to room temp and cooled again).
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