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Old 01-28-2020, 06:36 AM   #1
Location: UK

Join Date: Oct 2010
Posts: 21
Default 1000 barcodes

Has anybody got any experience in pooling 1000 barcodes?
I am planning a nested multiplex PCR, testing several loci in 1000 clonal colonies for indels. Second PCR should introduce barcodes. IDT have a list, but before I go picking, is there anything I need to consider for compatibility? Has anyone optimised this type of thing?
mboth is offline   Reply With Quote

crispr, indexes, pcr primers

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