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Old 03-21-2020, 09:46 AM   #1
Shahrukh
Junior Member
 
Location: Russia

Join Date: Oct 2019
Posts: 3
Lightbulb Can I use secondary antibody as my negative IP control in my ChIP seq experiment?

I am doing a ChIP experiment for H3K9me3 marks in HEK293T cells. We have the ChIP grade Ab for H3K9me3 but didn't order ab for isotype control. We have some secondary abs accessible can we use them to control for IP?
If yes, which one from the following would be a better IGG control, by better I meant that will give us the minimum background for our ChIP?
a) Alexa FluorŪ 568 Goat Anti-Rabbit IgG Antibody (polyclonal)
b) Alexa FluorŪ 488 Goat anti-mouse IgG (minimal x-reactivity) Antibody (polyclonal)
c) Alexa FluorŪ 488 Donkey anti-rabbit IgG (minimal x-reactivity) Antibody (polyclonal)

Thank you!
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