SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > The Pipeline



Similar Threads
Thread Thread Starter Forum Replies Last Post
Software Engineer, Oxford Nanopore Technologies Oxford Nanopore Industry Jobs! 0 10-13-2009 03:16 AM
Scientific Programmer, Oxford Nanopore Technologies, UK Oxford Nanopore Industry Jobs! 0 05-05-2009 06:13 AM
Opportunities at Oxford Nanopore Technologies, UK Oxford Nanopore Industry Jobs! 0 03-05-2009 07:52 AM
Opportunities at Oxford Nanopore Technologies, UK Oxford Nanopore Industry Jobs! 6 12-10-2008 05:27 AM

Reply
 
Thread Tools
Old 10-22-2008, 09:05 AM   #1
mccullou
Member
 
Location: CA

Join Date: Oct 2008
Posts: 13
Default Oxford Nanopore

For originality this is the first 3rd gene sequencing by destruction. An exonuclease feeds cleaved bases into a modified hemolysin, while the potential is monitored. According to Oxford modified hemolysin (more specifically the cyclodextrin modification) interacts differently with each base resulting in a different potential drop allowing the base discrimination.

Pros: Look ma, no label. No amplification, no label, this is a close to pure DNA readout as one can imagine (might also do methylated DNA)
Con: Lipid bilayers are notoriously fluid causing motion in the pore. Manufacturing and kitting lipid bilayers as consumables, logistical nightmare. This is a great technology for a single pore, but pore arrays for this readout and the bilayer will be another large hurdle. Commercialization will be VERY challenging, perhaps this would be better as a service?

I will be interested to see how they progress.

Video here:
http://www.nanoporetech.com/sequences/
mccullou is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 07:58 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2017, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO