Go Back   SEQanswers > Bioinformatics > Bioinformatics

Similar Threads
Thread Thread Starter Forum Replies Last Post
Which kit for PCR products SylvainL Illumina/Solexa 1 02-05-2015 09:42 AM
Balance many PCR products for pooling Marius Sample Prep / Library Generation 4 05-02-2014 06:21 PM
Normalization of 16S PCR products cmazzoni Metagenomics 0 07-21-2011 06:17 AM
Sequencing PCR products windhorse8 Introductions 0 05-27-2011 12:27 PM
Sequencing for PCR products of different sizes deepakpatilp Illumina/Solexa 0 10-25-2010 08:15 AM

Thread Tools
Old 03-01-2018, 09:27 AM   #1
Junior Member
Location: Australia

Join Date: Mar 2018
Posts: 1
Default Poor PCR products

Hi everyone,

Actually, i am working on gene expression project and aimed to use RT-PCR and NGS methods. The NGS method work well BUT the PCR product for RT-PCR method was poor.

I designed the primers using primer-BLAST and then run PCR to check the presence and absence of the product. I run the gel but there was no products/no amplifications. I tried other primers but still no products. My target sequences have a lots of low complexity regions, and based on what i read the low complexity regions consume the PCR product and caused the failure PCR (no products). So I concentrated on the NGS method only instead of using RT-PCR. My question is this enough to justify the reason of excluded the RT-PCR method cause of the low complexity regions which is led to the failure PCR or i should try other primers until its work ?? Or there is another possible reasons to get this failure PCR?

loly is offline   Reply With Quote

poor pcr products

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 10:26 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO