Dear all,
looking up the FDA submission (510K) of the MiSeq Dx platform document, it is stated that the "Recommended minimal coverage per amplicon needed for accurate variant calling (Q(max_gt | poly_site) >=100) is 75x:
My questions are
1 - Is there a known rational for this 75x number?
2 - What does "Q(max_gt | poly_site) >=100" mean?
Thanks!
looking up the FDA submission (510K) of the MiSeq Dx platform document, it is stated that the "Recommended minimal coverage per amplicon needed for accurate variant calling (Q(max_gt | poly_site) >=100) is 75x:
My questions are
1 - Is there a known rational for this 75x number?
2 - What does "Q(max_gt | poly_site) >=100" mean?
Thanks!
Comment