Hi, fellows!
I would like to ask for your help concerning to the execution of TopHat.
I am working with reads from mRNA generated by a MiSeq machine.
Although this software had been developed for analysis of splice junctions, would it be a good idea use as a reference file a multifasta of transcripts already characterized? Or it would be better to use the wgs genome files?
Since now, thanks
I would like to ask for your help concerning to the execution of TopHat.
I am working with reads from mRNA generated by a MiSeq machine.
Although this software had been developed for analysis of splice junctions, would it be a good idea use as a reference file a multifasta of transcripts already characterized? Or it would be better to use the wgs genome files?
Since now, thanks
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