I am new to this forum, so I apologize if someone has asked this question already. I have been prepping total RNA from rat liver tissue using the miRNeasy kit from Qiagen and analyzing the RNA using the Agilent pico kit. My RIN values are averaging in the 2.3 range and I understand that the RIN value should be ~8. I was just wondering if others have used the same method of total RNA isolation from flash frozen tissue and what RIN values you typically see? Thanks for any help!
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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