hey. everyone! i have a sample containning many kinds of different cDNA, and i want to know their sequence and abundance in the sample. the problem is that the amount of the cDNA in the sample is limited. and i don't want to amplify them as this might introduce PCR bias, thus altering their relative abundance. can anyone tell me the minimal amount of DNA required for the next-generation sequencing? thanks a lot!
hanjay
hanjay
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