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Old 08-21-2016, 11:26 AM   #1
Location: Sweden

Join Date: Nov 2014
Posts: 18
Default Please help! DNA samples for whole genome sequencing

Hi folks,

I have extracted DNA from fungi for whole genome sequencing (Truseq PCR free with 350 bp insert and HiSeq 2500 PE) using a kit. The extraction went smooth until I realized at the end that I treated my samples with only half of the recommended amount for RNase A (2 ul of 10 mg/ml instead of 2 ul of 20 mg/ml). I tried checking the DNA samples on 1% agarose and following is the gel image with 100-1000 bp ladder.

I am not sure there are RNA bands (very faint though) at around 100-200 bp. Do you think my DNA samples are good enough to go for whole genome sequencing without additional treatments? Or shall I try to treat them with RNase A again? Unfortunately, I run out of fresh samples.

I appreciate all your comments and suggestions.

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Old 08-21-2016, 11:40 AM   #2
josh kinman
Location: Austin

Join Date: Apr 2014
Posts: 71

These look fine. Even if you have some RNA in your sample it won't make into your final library after fragmentation and ligation steps.
jdk787 is offline   Reply With Quote

dna extraction, rna dna contamination, whole genome sequencing

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