Go Back   SEQanswers > Bioinformatics > Bioinformatics

Similar Threads
Thread Thread Starter Forum Replies Last Post
Agilent SureSelect exome enrichment bed file yl01 Bioinformatics 8 12-15-2013 03:45 AM
Agilent SureSelect Kit ssing General 1 08-02-2012 11:42 AM
Agilent SureSelect: problems with target enrichment Asereth SOLiD 6 06-05-2012 11:37 PM
Insert size with Agilent SureSelect sdavis Sample Prep / Library Generation 12 05-14-2010 11:39 AM
Agilent SureSelect enrichment javier Sample Prep / Library Generation 1 03-30-2010 12:39 PM

Thread Tools
Old 05-15-2013, 02:00 AM   #1
Location: Oxford

Join Date: Nov 2010
Posts: 24
Default Agilent SureSelect bed files


Can anyone tell me what the difference is between these bed files supplied by Agilent for their SureSelect Human All Exon V2 kit:


Kath is offline   Reply With Quote
Old 07-20-2013, 08:00 AM   #2
Senior Member
Location: UK

Join Date: Jan 2010
Posts: 390

The padded bed file is the regions that are baited, with an extension of 50 bases to either end. Where this regions meet they are merged into a single region.

These tend to extend into the intronic regions (and generally defines what you can confidently genotype).

Regions is the regions the kit is designed to (I think). Covered is the regions that are baited for capture.
Bukowski is offline   Reply With Quote
Old 09-10-2013, 03:53 PM   #3
Location: Australia

Join Date: Mar 2011
Posts: 85

Very helpful, thanks.
Elsie is offline   Reply With Quote

bedfile, exome capture

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 08:42 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO