Go Back   SEQanswers > General

Similar Threads
Thread Thread Starter Forum Replies Last Post
Concentration of lambda dna Shiwali Goyal General 2 09-08-2015 01:46 AM
Low DNA concentration cngl Sample Prep / Library Generation 0 08-11-2015 06:19 AM
DNA concentration with Ampure? jdp Sample Prep / Library Generation 1 11-11-2014 05:35 AM
DNA concentration increased MonaE Ion Torrent 19 09-05-2013 10:49 AM
help regarding starting concentration of dna balaji_chattopadhyay Illumina/Solexa 1 06-21-2012 10:35 AM

Thread Tools
Old 09-24-2020, 01:46 AM   #1
Junior Member
Location: Southeast Asia

Join Date: Apr 2019
Posts: 4
Default Why is my DNA concentration and absorbance ratios fluctuating?

I have done DNA extraction on human saliva using Promega ReliaPrep™ Blood gDNA Miniprep System that have been expired for more than five years now. When I initially checked the DNA concentration and the absorbance ratio, it shows a decent DNA concentration (~100 ng/ul) and decent absorbance ratios (A260/A280 = ~1.8 and A260/A230 = ~2.2). When I checked the DNA concentration and absorbance ratio a second time sometimes within minutes to days from the first check, the DNA concentration and absorbance ratio either drops or increases wildly.

For every nanodrop and qubit measurements, I have vortexed the tube first. For nanodrop specifically, I have used 2 µL and make sure to eliminate air bubbles.

Why is there many fluctuations in DNA concentration and absorbance ratios on my salivary human DNA samples?

Apologies if this is the wrong place to ask.
sidolfus is offline   Reply With Quote

dna extraction, nanodrop, qubit

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 10:30 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO