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  • STAR ERROR: not enough memory for BAM sorting

    Dear all,

    I am struggling to get this command right, and getting errors all the time.

    First Error:
    $ STAR --genomeDir ../Genome/GenomeDir/ --readFilesIn xxx_R1_L001.fastq.gz xxx_R2_L001.fastq.gz --readFilesCommand zcat --outFileeName Prefix xxx --outSAMtype BAM SortedByCoordinate

    EXITING because fatal ERROR:not enough memory for BAM sorting.
    SOLUTION: re-rrun STAR with at least --limitBAMsortRAM 123762737

    I included the option in my command and this is what I got... and then
    Second Error:
    $ STAR --limitBAMsortRAM 123762737 --genomeDir ../Genome/GenomeDir/ --readFilesIn xxx_R1_L001.fastq.gz xxx_R2_L001.fastq.gz --readFilesCommand zcat --outFileeName Prefix xxx --outSAMtype BAM SortedByCoordinate

    EXITING because of FATAL ERROR: failed reading from temporary file: xxx_STARtmp//BAMsort/0/43

    Can somebody help me out whit this? I have literally lost hope

    Thank you in advance
    Attached Files

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