Hello everybody,
can anyone tell me whether the concentration of P1 beads within P1 reagent (Box 2, SOLiD EZ Bead Emulsifier) is the same as that of P1 beads of ePCR Kit V2, that should be 10,000,000 beads /ul? As far as we use the EZ Bead system and not the IKA shaker we´ve got only 3 boxes for emulsifier (P1 beads included for DNA enrichment and not separate P1 beads for calculation of enriched P1beads conc.). Can I prepare the calibration curve from the remaining P1 beads assuming 10 mil.beads/ul concentration, too?thanks.
can anyone tell me whether the concentration of P1 beads within P1 reagent (Box 2, SOLiD EZ Bead Emulsifier) is the same as that of P1 beads of ePCR Kit V2, that should be 10,000,000 beads /ul? As far as we use the EZ Bead system and not the IKA shaker we´ve got only 3 boxes for emulsifier (P1 beads included for DNA enrichment and not separate P1 beads for calculation of enriched P1beads conc.). Can I prepare the calibration curve from the remaining P1 beads assuming 10 mil.beads/ul concentration, too?thanks.
Comment