Hi all,
I am a new member of this RNAseq forum and a first year PhD student. I want to look at lncRNA profiling using RNAseq (illumuna hiseq 2000).
Any general advice would be very helpfull but I also have some specific questions:
1) How deep I have to sequence to find them, i.e.how many reads per sample?
2) paired end sequencing: I know what a paired end read it but why is it better? Is it just the coverage that is improved?
thanks in advance
grts
I am a new member of this RNAseq forum and a first year PhD student. I want to look at lncRNA profiling using RNAseq (illumuna hiseq 2000).
Any general advice would be very helpfull but I also have some specific questions:
1) How deep I have to sequence to find them, i.e.how many reads per sample?
2) paired end sequencing: I know what a paired end read it but why is it better? Is it just the coverage that is improved?
thanks in advance
grts
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