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  • Simple unix command to add dummy barcodes to fastq

    Hi,

    Little out of practice even with my most basic unix skills.
    No doubt it is a simple solution.

    I want to add a dummy barcode e.g. AGACTGA to the start of every sequence in a fastq file. I would then need to add the same length of a quality value to the quality information.

    Could someone help me with a most likely simple unix command that I could use in a shell script to add unique dummy data to the corresponding lines in unique fastq files.

  • #2
    Code:
    zcat somefile.fq.gz | awk '{if(NR%4==2) {printf("AGACTGA%s\n",$0)} else if(NR%4 == 0) {printf("IIIIIII%s\n", $0)} else {print $0}}' | gzip > somefile.modified.fq.gz

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    • #3
      Many thanks, Devon!!

      J

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