Illumina provides a human.49.splice ELAND_RNA_GENOME_SPLICE file for 50bp reads. Does someone have the same file for 75bp reads?
There is this thought that 75bp reads are too long for mrna-seq, as they may cover more than one splice sites, thus causing more harm than help. Do people have comments on that? We have 75 bp reads and wish to use the Illumina Genome Studio pipeline but do not have access to the 75bp splice file.
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There is this thought that 75bp reads are too long for mrna-seq, as they may cover more than one splice sites, thus causing more harm than help. Do people have comments on that? We have 75 bp reads and wish to use the Illumina Genome Studio pipeline but do not have access to the 75bp splice file.
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