Reagent v3 Miseq stability

[alejandra13]

Hello everyone!

I left reagent V3 in water bath at room temperature for roughly 3 hours. I got a low Q30 score and the cluster density was 1050 K/mm2.

Do you think that the thawing process could affect?

Thanks for your answers!

[Saravanan]

I kept the reagent cartridge in water bath for almost 2 hr and it was OK.
The cluster density 1050 K/mm2 is in normal range.
I think the quality of your sample might not good that's why you got low Q30 score. So, I don't think 3 hrs thawing might affect your seq.

Can you share your bioanalyzer result?