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I'm designing some qPCR reactions to test some candidate genes before we get our RNA-seq results back. Does anyone have a good suggestion for a control gene to use for bacterial samples? I've heard that just using 16S rRNA is problematic because it is so abundant. Also, since rRNA shouldn't be in my RNA-seq reactions, I'm hesitant to use that as a control. I've seen a lot of people use recA. Is this common, or are there other suggestions?
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
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