Hi
following is the results of samtools flagstat results. I use bwa mem default command to do align! my question is why the properly paired is odd number?
25700926 mapped
25744459 + paired in sequencing
12897523 read1
12846936 read2
25445603 properly paired
25692729 with itself and mate mapped
8197 singletons
my reads are from a archaea RNA-seq experiment.
and how to get unique mapped read-pairs from the properly paired?
thanks!
following is the results of samtools flagstat results. I use bwa mem default command to do align! my question is why the properly paired is odd number?
25700926 mapped
25744459 + paired in sequencing
12897523 read1
12846936 read2
25445603 properly paired
25692729 with itself and mate mapped
8197 singletons
my reads are from a archaea RNA-seq experiment.
and how to get unique mapped read-pairs from the properly paired?
thanks!
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