Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • barcode library for ion torrent

    Hi everybody, I am new in the NGS field. I want to ask a question. If I want to barcode my samples, should I do paired barcode or one side barcode, considering the reading length of ion torrent, If the fragment is more than 300bp, maybe there will be an issue to do paired barcod?

    I want to do a pilot experiment to test my propose. but I hardly find literature regarding to my question.

    I wonder if any of you have experience on this, can you share your thoughts?

    Greatly appreciated!

  • #2
    One barcode because Ion Torrent software doesn't look for barcodes on the other ends.

    Comment


    • #3
      Originally posted by maven View Post
      One barcode because Ion Torrent software doesn't look for barcodes on the other ends.
      thanks for your reply,
      Another question is, I am not sure in which end to put barcode, in the beads end, is it P1 adapter and the other is A adpater? If I want to add barcode for sucessful read through, which end is better.

      Thanks a lot

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Techniques and Challenges in Conservation Genomics
        by seqadmin



        The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

        Avian Conservation
        Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
        03-08-2024, 10:41 AM
      • seqadmin
        The Impact of AI in Genomic Medicine
        by seqadmin



        Artificial intelligence (AI) has evolved from a futuristic vision to a mainstream technology, highlighted by the introduction of tools like OpenAI's ChatGPT and Google's Gemini. In recent years, AI has become increasingly integrated into the field of genomics. This integration has enabled new scientific discoveries while simultaneously raising important ethical questions1. Interviews with two researchers at the center of this intersection provide insightful perspectives into...
        02-26-2024, 02:07 PM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 03-14-2024, 06:13 AM
      0 responses
      34 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-08-2024, 08:03 AM
      0 responses
      72 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-07-2024, 08:13 AM
      0 responses
      82 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-06-2024, 09:51 AM
      0 responses
      68 views
      0 likes
      Last Post seqadmin  
      Working...
      X