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Old 01-10-2011, 11:03 AM   #1
madsaan
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Location: california

Join Date: Jan 2011
Posts: 26
Default fastx newbie

Hi,

I am new to NGS analysis .I am currently trying to understand the output of the fastx quality stats for my sequenced transcriptome .There is a column 'mean' which is the Mean quality score value for a particular column.Is this the value I should consider when I am faced with the question of trimming the bases whose cycle quality score is less.Also,what is considered a bad quality score.is it anything less than 20?


Thanks,
Joji.
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