Hi, everyone. I am now working on NGS preprocessing of data. When I checked GEO for illumina platform information. I can find that some of the data used Illumina Genome Analyzer II and some just used Illumina Genome Analyzer. Since I am doing quality control, I need to be full aware of the correspondent encoding methods, namely, ASCII+33 and ASCII+64. I know that 64 is for Illumina 1.5+ and 33 is for Illumina 1.9+. I was wondering if Illumina Genome Analyzer II is using 33 and Illumina Genome Analyzer is using 64. Thanks for answering this stupid question.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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