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Old 05-22-2017, 05:30 AM   #1
sfranzenburg
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Default Bacterial Genome in a Bottle

Dear All,

I am searching for the bacterial equivalent of the human "Genome in a Bottle", meaning a commercially available bacterial reference DNA to assess Sequencing Performance.
Is there such a thing that just everybody uses? I found the RM 8375 but it is out of stock / discontinued (?).

best,
Sören
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Old 05-22-2017, 05:48 AM   #2
thermophile
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Are you looking for a mixed community? zymo has a couple.
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Microbial ecologist, running a sequencing core. I have lots of strong opinions on how to survey communities, pretty sure some are even correct.
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Old 05-23-2017, 06:18 AM   #3
sfranzenburg
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I just need once single strain to establish and assess performance of the MinION in our lab.
I think I will just go for e.coli K12.
Just figured I'd ask if there is any gold standard.
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