Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Unable to see BAM files from CLC on geneious or IGV

    We got BAM files from our service provider on CLC workbench. However, it is not possible to visualize them on both geneious and IGV for some reason. Importation fails in Geneious. I think the issue is with the reference genome. It seems like the FASTA file is not recognised due to some season as being the same one as used for the mapping my CLC. Any suggestions are welcome.

    I have sorted the BAM files on SAMTools and generated the bai files, still no results
    Last edited by vl80; 07-15-2014, 10:49 PM.

  • #2
    Probably stating the obvious here. Ask the provider for the original fastq files or the reference fasta they used to generate the BAM's.

    Or you could look in the BAM by using "samtools view -h <in.bam> > <out.sam>" to see if it just a matter of having to appropriately rename the ID's in reference fasta (e.g. chrX instead of X) you are using so they match.
    Last edited by GenoMax; 07-16-2014, 02:44 AM.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Essential Discoveries and Tools in Epitranscriptomics
      by seqadmin




      The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
      Yesterday, 07:01 AM
    • seqadmin
      Current Approaches to Protein Sequencing
      by seqadmin


      Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
      04-04-2024, 04:25 PM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 04-11-2024, 12:08 PM
    0 responses
    57 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 10:19 PM
    0 responses
    53 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 09:21 AM
    0 responses
    45 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-04-2024, 09:00 AM
    0 responses
    55 views
    0 likes
    Last Post seqadmin  
    Working...
    X