Hello,
I just want to know. During the TruSeq v2 sample preparation workflow, the first step is purify and fragment mRNA. My question is how long this fragmentation method will produce? Is the length same or different for each mRNA? Is there any way to know how long per fragment as a result of this procedure from FASTA/FASTQ/SAM file? Thank you for your answer.
I just want to know. During the TruSeq v2 sample preparation workflow, the first step is purify and fragment mRNA. My question is how long this fragmentation method will produce? Is the length same or different for each mRNA? Is there any way to know how long per fragment as a result of this procedure from FASTA/FASTQ/SAM file? Thank you for your answer.
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