Hi all,
I heard that we cannot do only 2 samples (2 barcodes) in one lane of the flow cell, and that it is better to have 6 samples per lane. Is it true?
The explanation I got is that if there are only 2 indexes, half of the clusters will give the same signal, when the sequencing reaction reach the barcode area. And this will create problem for the machine to identify individual clusters.
I am quite confused about this issue.
I would appreciate it a lot if you can share your experience/thoughts on this in layman's terms
Ken
I heard that we cannot do only 2 samples (2 barcodes) in one lane of the flow cell, and that it is better to have 6 samples per lane. Is it true?
The explanation I got is that if there are only 2 indexes, half of the clusters will give the same signal, when the sequencing reaction reach the barcode area. And this will create problem for the machine to identify individual clusters.
I am quite confused about this issue.
I would appreciate it a lot if you can share your experience/thoughts on this in layman's terms
Ken
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