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  • Transitioning from Previous-Gen to Next-Gen

    Hello, everyone.

    I learned about this site from reading Keith Robison's Omics Omics blog. This site looks like it will be an excellent resource.

    I work at a large biotech company. Our small satellite site has a single ABI sequencer, and I'm very experienced at previous-generation sequencing. My colleagues at our headquarters are experimenting with different next-generation sequencing platforms, and I anticipate that we'll switch over to next-gen sequencing in one to two years.

    I rely heavily on BioPerl, and I'm a subscriber to the BioPerl mailing lists. I see that the core developers are beginning to tackle support for next-generation sequencing. I hope to learn from comments here what additional software is being written for managing and analyzing next-generation sequencing.

    I plan to attend ISMB 2010 this summer, where it appears there will be sessions and a satellite meeting devoted to next-gen sequencing. Is anyone else going?

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    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
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