Hello,
I hope someone here can help with this rather weird problem we are having.
For a recent project, we seqenced the metagenome from several patients using the IonTorrent Proton plateform. The results obtained while analyzing these sequences with Metaphlan2 are quite puzzling.
The only identification that Metaphlan provides is some unnamed viruses... kViruses|pViruses_noname|cViruses_noname|oViruses_noname|fViruses_noname|gViruses_noname|sChilli_leaf_curl_Multan_alphasatellite|tPRJNA39933
I know my samples have a high human DNA contamination level, so I removed most of them using Deconseq. However that didn't improve the results, I still have the same weird viruses as only results.
Is there a known issue of Metaphlan with Proton sequences ? Or is there just too many human sequences in my sample ?
Thanks !
I hope someone here can help with this rather weird problem we are having.
For a recent project, we seqenced the metagenome from several patients using the IonTorrent Proton plateform. The results obtained while analyzing these sequences with Metaphlan2 are quite puzzling.
The only identification that Metaphlan provides is some unnamed viruses... kViruses|pViruses_noname|cViruses_noname|oViruses_noname|fViruses_noname|gViruses_noname|sChilli_leaf_curl_Multan_alphasatellite|tPRJNA39933
I know my samples have a high human DNA contamination level, so I removed most of them using Deconseq. However that didn't improve the results, I still have the same weird viruses as only results.
Is there a known issue of Metaphlan with Proton sequences ? Or is there just too many human sequences in my sample ?
Thanks !
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