I have experienced some problems in the lab recently, and I am interested if anybody has any comment or suggestions.
We have noticed lower than usual yields for library preps. Now some of the data has come back, and there is a high level of duplication in the samples. I would normally expect this from a sample which has been over-amplified.
The problem first occurred in RNA-seq libraries, but now also does in any other kind of library prep we do.
I am thinking that something in my lab is degrading RNA and DNA alike as I am preparing the libraries.
My number one suspect is the DNA Zap solutions that I frequently use in order to avoid cross-contamination of projects.
Has anybody ever seen this happen? How potent is the DNA Zap? What are other people using?
I appreciate any comment you might have.
We have noticed lower than usual yields for library preps. Now some of the data has come back, and there is a high level of duplication in the samples. I would normally expect this from a sample which has been over-amplified.
The problem first occurred in RNA-seq libraries, but now also does in any other kind of library prep we do.
I am thinking that something in my lab is degrading RNA and DNA alike as I am preparing the libraries.
My number one suspect is the DNA Zap solutions that I frequently use in order to avoid cross-contamination of projects.
Has anybody ever seen this happen? How potent is the DNA Zap? What are other people using?
I appreciate any comment you might have.
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