Hi,Hello,
I am using 150ng FFPE DNA to prepare a library for HiSeq. After 1st amplification, DNA concentrations are too low, not enough for 500ng suggested for hybridisation. Followed the protocol excatly.
Any advise appreciated. Should I increase PCr cycles from 10 to 12 ?? But with Sure Select Agilent kits we haev seen that increasing PCr cycles decreases conc of DNA.
Thanks
I am using 150ng FFPE DNA to prepare a library for HiSeq. After 1st amplification, DNA concentrations are too low, not enough for 500ng suggested for hybridisation. Followed the protocol excatly.
Any advise appreciated. Should I increase PCr cycles from 10 to 12 ?? But with Sure Select Agilent kits we haev seen that increasing PCr cycles decreases conc of DNA.
Thanks
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