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  • #1

    Werid RNA traces- bump above 28S - can I make libraries from this?

    Hi all,
    I have traces from a fragment analyzer (very similar to bioanalyzer; basically your capillary gel run) and am getting some weird traces. Some (well A2, A6 and A7 for example) have things higher than 28S - any idea what these can be? Also - A6 and A7 have very streange humps instead of just two distinct peaks. Can I make libraries out of these?
    Thanks
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  • #2
    Hi Noa-

    My guess is genomic DNA contamination. Did you DNase treat it when on the column?

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