|
|
|
|
|||||||
Similar Threads
|
||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Time & Cost of using 1 MiSeq Machine to do 16s rDNA (V2/V4) Seq on 300 Samples/Month | vs92 | Illumina/Solexa | 28 | 10-09-2015 11:07 AM |
| Miseq Custom primer for 16s V1V3 region | lmma | Illumina/Solexa | 12 | 02-26-2015 01:13 PM |
| 454 vs MiSeq - Need to optimize time/cost for 16s analysis of 300 Stool Samples/month | vs92 | Metagenomics | 15 | 12-01-2012 06:35 AM |
| Quantitative 16S profiling with multiple 16S genes per genome | AaronS | Metagenomics | 1 | 10-26-2012 12:16 PM |
| 16S metagenomics, how much data needed? | Gorbenzer | Metagenomics | 3 | 05-13-2012 06:43 PM |
 |
|
|
Thread Tools |
11-21-2012, 01:10 PM
|
#1 |
|
Member
Location: Earth Join Date: Jul 2012
Posts: 13
|
Who uses a MiSeq for 16S data?
Hi Everyone,
I'm interested to hear about anyone's experiences with 16S sequencing on the MiSeq. I'm aware of the papers by Caporaso, Illumina's tech notes, methods for 'hard-coding' matrix and phasing values, etc. What I'm interested in is actual experiences: What methods are you using for primer design and indexing? How many indexes are you running per lane? What kind of quality are you getting? What kind yield? Etc. Cheers! |
|
|
|
| Thread Tools | |
|
|
