Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • NovaSeq metatranscriptomics

    Hi all,

    I was wondering if anyone has experience with (a) metatranscriptomics run(s) on the NovaSeq 6000?
    Our preferred company switched from HiSeq to NovaSeq. We are planning to sequence about 110 soil samples.
    Some years ago, we used the BiooScientific NEXTflex™ Rapid Directional mRNA-Seq Kit for a HiSeq 2500 run.
    I guess NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0 or the NEXTFLEX® Rapid Directional qRNA-Seq™ Library Prep Kit are the current incarnations. Are these compatible with NovaSeq? Are there alternatives you could suggest?

    Thanks!

    Best regards

  • #2
    Does anybody have any suggestions?

    In addition, is it possible to multiplex >100 samples on a NovaSeq using existing tags?

    thanks

    Comment


    • #3
      Yes it is possible to do 384 (and many more if you do custom designs).

      The IDT® for Illumina®-Nextera™ DNA UD Indexes Sets A, B, C, and D Indexes offer up to 384 unique dual indexing that enables accurate assignment of reads and efficient use of the flow cell. These unique dual index codes use 10 base pair codes.
      See this page.

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Strategies for Sequencing Challenging Samples
        by seqadmin


        Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
        03-22-2024, 06:39 AM
      • seqadmin
        Techniques and Challenges in Conservation Genomics
        by seqadmin



        The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

        Avian Conservation
        Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
        03-08-2024, 10:41 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, Yesterday, 06:37 PM
      0 responses
      10 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, Yesterday, 06:07 PM
      0 responses
      9 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-22-2024, 10:03 AM
      0 responses
      50 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 03-21-2024, 07:32 AM
      0 responses
      67 views
      0 likes
      Last Post seqadmin  
      Working...
      X