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Old 02-28-2019, 11:10 AM   #1
materan96
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Location: Ecuador

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Default Demultiplex nanopore reads with custom barcodes

We have a problem trying to demultiplex MinION sequences with custom barcodes. Do you have any software recommendations we can try for demultiplexing or how to demultiplex these custom barcodes with Albacore? We have tried using albacore but it only recognizes barcodes provided by Nanopore Technologies.
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Old 02-28-2019, 03:25 PM   #2
GenoMax
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If you know the barcode sequences you may be able to use bbduk.sh from BBMap suite to bin the reads you are interested in.
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Old 03-05-2019, 04:49 AM   #3
Jugsy67
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Default re Minion and multiples barcodes

Hi Guys did this work i.e. using bbduk.sh from BBMap to bin the barcoded reads? We want to do the same for 16S rRNA gene amplicons and 96 barcodes on the MinIon.
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Old 06-25-2019, 08:41 PM   #4
tickteng
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how about porechop?
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