Hi all,
I have aligned paired-end RNA-Seq data generated with a strand-specific library (on Illumina HiSeq).
Is possible to known how to assign the pairs to the RNA strand from which was generated from the SAM file?
In other word I am looking for a way to generate two different bigWig files: one with the signal coming from one strand, and one with the signal coming from the other strand..
Thanks very much!
Best,
albert
I have aligned paired-end RNA-Seq data generated with a strand-specific library (on Illumina HiSeq).
Is possible to known how to assign the pairs to the RNA strand from which was generated from the SAM file?
In other word I am looking for a way to generate two different bigWig files: one with the signal coming from one strand, and one with the signal coming from the other strand..
Thanks very much!
Best,
albert
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