Hi all,
I'm new to the forum and also new to NGS.
I would like your opinion on the approach to be followed for an experiment with the ION torrent.
The idea is to sequence the genome of the HCV virus (approximately 9000 bp) or at least 3 genes of the virus (each of about 1500 bp).
How it’s better to fragment the DNA into smaller sequences? Which could be the method to follow? Avoiding the sonication, how can I proceed with an enzymatic fragmentation? And which is the better way to design the primers?
Could anyone give me some ideas? I really appreciate it!
Thanks so much!
I'm new to the forum and also new to NGS.
I would like your opinion on the approach to be followed for an experiment with the ION torrent.
The idea is to sequence the genome of the HCV virus (approximately 9000 bp) or at least 3 genes of the virus (each of about 1500 bp).
How it’s better to fragment the DNA into smaller sequences? Which could be the method to follow? Avoiding the sonication, how can I proceed with an enzymatic fragmentation? And which is the better way to design the primers?
Could anyone give me some ideas? I really appreciate it!
Thanks so much!
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