Hello ! I would like to determine oligonucleotides of housekeeping (1) and target (2) genes with Primer3web.
I have these mRNA sequences :
(1) CTCCCGATCAGCAGCGTCTTATCTTCGCTGGCAAGCAACTTGAGGATGGTCGCACTCTCTCAGACTACAA
CATCCAGAAGGAATCCACTCTCCATCTCGTTCTCCGACTTCGTGGTGGTATGCAGAGTGTATTAAGATAT
AGATGGACAGTCCAATATTATTCTATATTTCTCATAAATGACAAGCAAGTCTATTATATTCTAAACAAAT
TAATGTGGCTTTATCTTATCAAGAGAAGAAGTGCACCCATCAATACAAGAAGAATCGTATCAAGATTGCA
GAG
(2) AACCTGAGCACCACCCCGTTTGGCCGTGCGCTGTATACCCTGAGCAGCGATACCCAGTGGATTGATCAGC
GTCTGGTGCATACCTTTAGCATGCTGCTGAACACCAGCGTGGCGGTGATTGCGAGCTTTATTGTGAACGC
GATTGTGAACGTGTATTTTATTATTTTTCTGATTCCGATTGGCTTTGTGTTTTTTGGCATTCTGGTGTAT
TTTGTGGCGACCAGCCGTATGCTGCAGCGTATTGAAAGCGTGACCCGTAGCCCGGTGTTTGCGCATTTTA
GCGAAACCCTGGGCGGCCTGCCGATTATTCGTGCGTATCGTGATGAAAAACGTTTTTTTAAAAACATTCT
GGCGAAAATTGATAAAAACAACACCGCGCTGGTGTATCTGTATACCGCGAACCGTTGGCTGGCGATTCGT
CTGGATTATCTGGGCGCGGTGATTGTGCTGGTGGCGAGCCTGAGCGCGCTGCTGGGCGCGTTTTATCTGG
GCGTGGATGCGAGCCTGGTGGGCCTGGCGATTACCTATAGCCTGGAAATTAGCATTTATATGAACATGGT
GGTGCGTGGCTGCGCGGATCTGGAACTGCAGATGAACGCGATGGAACGTATTCGTTATTATACCGAAGTG
CCGAACGAAGAATATGAAGGCCTGGAACCGCCGCCGGAATGGCCGCATAAAGGCGAAATTATTCTGGATA
ACATTAGCGTGCGTTATGCGGCGGAACTGGATCCGGTGCTGCCGCTGAGCGGCTATTTTAGCCCGGGCGG
CTTTGGCTGGTATAGCGTGCTGCATACCGGCAGCGGCAAAAGCAGCTTTACCCTGGCGCTGTATCAT
Can you help me to choose parameter values (see attach file) please ?
Thank you !
I have these mRNA sequences :
(1) CTCCCGATCAGCAGCGTCTTATCTTCGCTGGCAAGCAACTTGAGGATGGTCGCACTCTCTCAGACTACAA
CATCCAGAAGGAATCCACTCTCCATCTCGTTCTCCGACTTCGTGGTGGTATGCAGAGTGTATTAAGATAT
AGATGGACAGTCCAATATTATTCTATATTTCTCATAAATGACAAGCAAGTCTATTATATTCTAAACAAAT
TAATGTGGCTTTATCTTATCAAGAGAAGAAGTGCACCCATCAATACAAGAAGAATCGTATCAAGATTGCA
GAG
(2) AACCTGAGCACCACCCCGTTTGGCCGTGCGCTGTATACCCTGAGCAGCGATACCCAGTGGATTGATCAGC
GTCTGGTGCATACCTTTAGCATGCTGCTGAACACCAGCGTGGCGGTGATTGCGAGCTTTATTGTGAACGC
GATTGTGAACGTGTATTTTATTATTTTTCTGATTCCGATTGGCTTTGTGTTTTTTGGCATTCTGGTGTAT
TTTGTGGCGACCAGCCGTATGCTGCAGCGTATTGAAAGCGTGACCCGTAGCCCGGTGTTTGCGCATTTTA
GCGAAACCCTGGGCGGCCTGCCGATTATTCGTGCGTATCGTGATGAAAAACGTTTTTTTAAAAACATTCT
GGCGAAAATTGATAAAAACAACACCGCGCTGGTGTATCTGTATACCGCGAACCGTTGGCTGGCGATTCGT
CTGGATTATCTGGGCGCGGTGATTGTGCTGGTGGCGAGCCTGAGCGCGCTGCTGGGCGCGTTTTATCTGG
GCGTGGATGCGAGCCTGGTGGGCCTGGCGATTACCTATAGCCTGGAAATTAGCATTTATATGAACATGGT
GGTGCGTGGCTGCGCGGATCTGGAACTGCAGATGAACGCGATGGAACGTATTCGTTATTATACCGAAGTG
CCGAACGAAGAATATGAAGGCCTGGAACCGCCGCCGGAATGGCCGCATAAAGGCGAAATTATTCTGGATA
ACATTAGCGTGCGTTATGCGGCGGAACTGGATCCGGTGCTGCCGCTGAGCGGCTATTTTAGCCCGGGCGG
CTTTGGCTGGTATAGCGTGCTGCATACCGGCAGCGGCAAAAGCAGCTTTACCCTGGCGCTGTATCAT
Can you help me to choose parameter values (see attach file) please ?
Thank you !
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