Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Primer3 : oligonucleotide determining

    Hello ! I would like to determine oligonucleotides of housekeeping (1) and target (2) genes with Primer3web.
    I have these mRNA sequences :
    (1) CTCCCGATCAGCAGCGTCTTATCTTCGCTGGCAAGCAACTTGAGGATGGTCGCACTCTCTCAGACTACAA
    CATCCAGAAGGAATCCACTCTCCATCTCGTTCTCCGACTTCGTGGTGGTATGCAGAGTGTATTAAGATAT
    AGATGGACAGTCCAATATTATTCTATATTTCTCATAAATGACAAGCAAGTCTATTATATTCTAAACAAAT
    TAATGTGGCTTTATCTTATCAAGAGAAGAAGTGCACCCATCAATACAAGAAGAATCGTATCAAGATTGCA
    GAG
    (2) AACCTGAGCACCACCCCGTTTGGCCGTGCGCTGTATACCCTGAGCAGCGATACCCAGTGGATTGATCAGC
    GTCTGGTGCATACCTTTAGCATGCTGCTGAACACCAGCGTGGCGGTGATTGCGAGCTTTATTGTGAACGC
    GATTGTGAACGTGTATTTTATTATTTTTCTGATTCCGATTGGCTTTGTGTTTTTTGGCATTCTGGTGTAT
    TTTGTGGCGACCAGCCGTATGCTGCAGCGTATTGAAAGCGTGACCCGTAGCCCGGTGTTTGCGCATTTTA
    GCGAAACCCTGGGCGGCCTGCCGATTATTCGTGCGTATCGTGATGAAAAACGTTTTTTTAAAAACATTCT
    GGCGAAAATTGATAAAAACAACACCGCGCTGGTGTATCTGTATACCGCGAACCGTTGGCTGGCGATTCGT
    CTGGATTATCTGGGCGCGGTGATTGTGCTGGTGGCGAGCCTGAGCGCGCTGCTGGGCGCGTTTTATCTGG
    GCGTGGATGCGAGCCTGGTGGGCCTGGCGATTACCTATAGCCTGGAAATTAGCATTTATATGAACATGGT
    GGTGCGTGGCTGCGCGGATCTGGAACTGCAGATGAACGCGATGGAACGTATTCGTTATTATACCGAAGTG
    CCGAACGAAGAATATGAAGGCCTGGAACCGCCGCCGGAATGGCCGCATAAAGGCGAAATTATTCTGGATA
    ACATTAGCGTGCGTTATGCGGCGGAACTGGATCCGGTGCTGCCGCTGAGCGGCTATTTTAGCCCGGGCGG
    CTTTGGCTGGTATAGCGTGCTGCATACCGGCAGCGGCAAAAGCAGCTTTACCCTGGCGCTGTATCAT
    Can you help me to choose parameter values (see attach file) please ?
    Thank you !
    Attached Files

  • #2
    Please don't post the same question in multiple forums. I have deleted the duplicates.

    Comment


    • #3
      Sorry for the duplicates. This is the first time I use this forum and I did not known which place between "RNA Sequencing", "General" and "Bioinformatics" was the best.
      Thank you for you comprehension.

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Essential Discoveries and Tools in Epitranscriptomics
        by seqadmin




        The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
        04-22-2024, 07:01 AM
      • seqadmin
        Current Approaches to Protein Sequencing
        by seqadmin


        Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
        04-04-2024, 04:25 PM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, Today, 08:47 AM
      0 responses
      12 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-11-2024, 12:08 PM
      0 responses
      60 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 10:19 PM
      0 responses
      59 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 09:21 AM
      0 responses
      54 views
      0 likes
      Last Post seqadmin  
      Working...
      X