Hi everyone,
I noticed a strange effect on our HiSeq - the lower intensity of A and C on left side of the swath (attached are two thumbnails illustrationg this).
This effect is not flowcell- or library-dependent - I ran several flowcells, PE and SR, with different libraries, and it is always the same thing.
The fact that this concerns only A and C nucleotides makes me think that something is wrong with the laser. However our FSE who checked the lasers recently says that they are OK.
Has anyone faced with similar problem?
I noticed a strange effect on our HiSeq - the lower intensity of A and C on left side of the swath (attached are two thumbnails illustrationg this).
This effect is not flowcell- or library-dependent - I ran several flowcells, PE and SR, with different libraries, and it is always the same thing.
The fact that this concerns only A and C nucleotides makes me think that something is wrong with the laser. However our FSE who checked the lasers recently says that they are OK.
Has anyone faced with similar problem?
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