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Old 01-21-2015, 11:30 AM   #1
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Location: Oregon

Join Date: Jan 2015
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Default Agalma pipeline only using a single thread for RAxML

Hello all,

I've been playing with the Agalma transcriptomics pipeline.

I'm attempting to run the genetree step in the pipeline, which involves running raxml on a large set of homologs clustered by mcl. Agalma then takes the individual gene trees and 'prunes' out paralogs.

Here's the problem: the genetree script works, but instead of executing multiple runs simultaneously, raxml is being run on one homolog at a time and only using a single thread despite having access to more. This isn't a huge issue on small datasets with few taxa, but it quickly becomes an insurmountable bottleneck.

I'd assumed there was a simple fix (Agalma is just calling raxml with bash) or some setting I'd overlooked, but after considerable exertion, I've yet to make any progress. The only thing I can think of doing is running raxml externally on all 7000+ homologs and inserting the trees back into Agalma, but I've got a feeling the SQL database won't like that.

Has anyone encountered this issue, or have any suggestions on solutions? Thanks!

I'm using a mac pro running Mavericks, and I get the same issue with a Ubuntu Linux 14.04 virtual machine.
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agalma, raxml

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