Tweet
What could be the reason for this bioanalyzer results for a total RNA stranded lib prep? I have been using the low mammalian input kit from clontech.
-
-
Could you post a higher-resolution version of this image and an electropherogram of the template RNA itself? This looks like it might be the result of degraded RNA. -
I see the same in my positive control which is suppose to be RNA of good quality.Comment
-
They could both be degraded if you had RNase contamination at some step. How do the RNAs look on the Bioanalyzer?Comment
-
What organism did the starting RNA material come from? A "spiky" RNA-seq library trace such as this often indicates the presence of high abundance transcripts. It is possible that your ribosomal depletion step was not very efficient.Comment
-
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
Comment