Tweet
I have two RNA ssRNA oligos that I have annealed into a dsRNA duplex. The concentration of the two ssRNAs were 10uM. What is then the concentration of the dsRNA duplex?
-
-
Really?
If you understand the definition of molarity, then the answer should be obvious (hint: consider the units for molarity). -
If you used equal volume of each oligo at 10 uM (without addition of any buffer), then the concentration of duplex would be 5 uM.Comment
-
Nucacidhunter, I was trying to teach a man how to fish...Comment
-
Hi HESmith
I understand your approach and agree with you. I just gave a fish for time being.Comment
-
Actually it could be 5uM or 10uM depending on what the OP meant by "The concentration of the two ssRNAs were 10uM."
It could mean that the + oligo was at 10uM and the - oligo was at 10uM and an equal amount of each was added to a tube to be annealed. Then the answer is 5uM.
It could mean that the solution of the tube containing the + and - oligo was at 10uM for each. That is 20uM ss-oligo concentration. Then the concentration is 10uM of duplex after they are annealed.
If you think the latter interpretation is ridiculous then what is the concentration of dATP in a 100mM dNTP solution?
--
PhillipComment
-
I agree with interpretations. To make it clear (I hope) that I assumed #1, my post was more than 3 characters.Originally posted by pmiguel View PostComment
-
The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...Yesterday, 07:48 AM -
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM
Comment