I use below PCR primers to prepare libraries for Miseq amplicon sequencing:
i7-INDEX2-Spacer- gene_specific_Primer
CAAGCAGAAGACGGCATACGAGAT TCGCCTTA GTCTCGTGGGCTCGG +gene_specific_Primer
i5-INDEX2-Spacer+gene_specific_Primer
AATGATACGGCGACCACCGAGATCTACAC-CTCTCTAT-TCGTCGGCAGCGTC+gene_specific_Primer
Compared to Nextera adapters, my primers do not include the tagmentation sequence. I tried to look up Miseq sequencing primers, but still not sure whether my adapters are compatible with Miseq sequencing primers.
Can I use default sequencing primers in Miseq cartridge for sequencing? Does anybody know any website or document to verify that?
i7-INDEX2-Spacer- gene_specific_Primer
CAAGCAGAAGACGGCATACGAGAT TCGCCTTA GTCTCGTGGGCTCGG +gene_specific_Primer
i5-INDEX2-Spacer+gene_specific_Primer
AATGATACGGCGACCACCGAGATCTACAC-CTCTCTAT-TCGTCGGCAGCGTC+gene_specific_Primer
Compared to Nextera adapters, my primers do not include the tagmentation sequence. I tried to look up Miseq sequencing primers, but still not sure whether my adapters are compatible with Miseq sequencing primers.
Can I use default sequencing primers in Miseq cartridge for sequencing? Does anybody know any website or document to verify that?
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