Tweet
I'm planning on sending out for sequencing soon using a MiSeq PE 250. I was wondering if its possible to use two Read 1 primers at once? I have a 16s sample that contains the PCR from two different primers (a general Bac and specific). Will running the sample with a Gen Bac Read 1, Specific Read 1, and their reverse Read 2 work? Or do I need to split them up into two seperate runs? I'm afraid it may cause interference.
-
Last edited by FNB; 07-16-2013, 06:40 PM. -
As a general principle it will work. The stansard practice for 16S amplicon sequencing was to spike in a large amount of PhiX to provide base balance. Custom primers are used for the 16S while standard primers for the PhiX, demonstrating that libraries using different sequencing primers can be mixed in one MiSeq run.Originally posted by FNB View Post
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
Comment