Hi All,
I'm interested in doing some transcriptome profiling to identify differentially expressed genes between two populations.
how much sequencing depth do I need to do a meaningful study? In the literature I've seen reports of 100 million reads on SOLID, 40-50 million on illumina, and down to 10 million 25bp reads on illumina?
Hoping to get some advice.
Thanks in advance
I'm interested in doing some transcriptome profiling to identify differentially expressed genes between two populations.
how much sequencing depth do I need to do a meaningful study? In the literature I've seen reports of 100 million reads on SOLID, 40-50 million on illumina, and down to 10 million 25bp reads on illumina?
Hoping to get some advice.
Thanks in advance
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