Hi everyone,
I am about to have SR run (for ChIP seq libraries) on HiScanSQ. I wonder what is the optimal cluster density for unbalanced samples like ChIP-seq libraries (a lot of the same DNA sequences) with a PhIX spike-in around 40 - 50%? Does anyone spike-in the PhIX control for ChIP-seq, instead of scarifying one lane for it?
I am about to have SR run (for ChIP seq libraries) on HiScanSQ. I wonder what is the optimal cluster density for unbalanced samples like ChIP-seq libraries (a lot of the same DNA sequences) with a PhIX spike-in around 40 - 50%? Does anyone spike-in the PhIX control for ChIP-seq, instead of scarifying one lane for it?
Comment