Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • SRA decryption help

    Dear all,

    I have a couple of *.sra.ncbi_enc files and I am trying to decrypt them; however, NCBI website is very confusing:

    1. Here they say that:

    The password is not given on the command line. It must be in a a file that should be able to be read only by the user account running the program.

    The location of that file should be found in one of these places in order:

    1. An environment variable VDB_PWFILE

    2. The VDB Configuration file option 'krypto/pwfile'
    2. Here they say:

    Decryption password and dbGaP repository key.

    Recently implemented “dbGaP repository key” is a dbGaP project wide security token required for running NCBI SRA toolkit and decryption tools. The key is provided in a file with suffix “.NGC”. Currently it needs to be downloaded from “My Research Projects” page of PI’s dbGaP account.

    3. Another Seqnaswers fellow told me to do this which did not work as well:

    1. make a new folder at your home directory: /home/xxx/.ncbi
    2. create a file named vdb-passwd and save your passwd in it
    3. run vdb-decrypt
    Any ideas?

    Thanks,
    GH

  • #2
    Watch this video: https://youtu.be/FjYO6Ys5cpc

    Comment


    • #3
      I get this error while decrypting by VDB-DECRYPT

      err: size "insufficient" while validating file size in file system module

      Comment


      • #4
        vdb-decrypt.2.9.0 err: size insufficient while validating file within file system module - cannot use filesize of zero when checking if cached file is complete

        Comment

        Latest Articles

        Collapse

        • seqadmin
          Current Approaches to Protein Sequencing
          by seqadmin


          Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
          04-04-2024, 04:25 PM
        • seqadmin
          Strategies for Sequencing Challenging Samples
          by seqadmin


          Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
          03-22-2024, 06:39 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by seqadmin, 04-11-2024, 12:08 PM
        0 responses
        25 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-10-2024, 10:19 PM
        0 responses
        29 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-10-2024, 09:21 AM
        0 responses
        25 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-04-2024, 09:00 AM
        0 responses
        52 views
        0 likes
        Last Post seqadmin  
        Working...
        X