Opentrons Automated Pipetting System

jhalpin · 06-15-2018, 10:27 AM

Has anyone here used the Opentrons OT-2 for library preparation? https://opentrons.com/ot-2

Bukowski · 06-15-2018, 03:10 PM

"Accuracy Accurate down to 384-well plates"

This is a new measure of accuracy that I was previously unaware of.

Their only 'Sequencing Application' in their protocol library is for 16S. There are liquid handling platforms with a much wider array of NGS protocols ready to go than this..

luc · 06-15-2018, 06:09 PM

A neighboring lab has the OT-One. This first version is unusable for just about anything - it can't hold the pipettes properly and the plates have a lot of play. Version two looks very different, though.

r.rosati · 06-18-2018, 06:45 AM

There have recently been a few discussions about them on Reddit; perhaps it might be a way to get in touch with people who use them.

luc · 03-13-2019, 06:38 PM

We recently got an OT2. So far it works for single channel pipetting.

We have problems with both of our multi-channel pipettes.
The magnetic module seems to have a design flaw - the magnet is too close to the samples, even when disengaged and at maximum distance (achieved with an self-made adapter). Thus, the beads do not stay in suspension for long - ideally they should stay in suspension for 5 minutes before then engaging the magnet.

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06-15-2018, 10:27 AM	#1
jhalpin Member Location: Atlanta, GA Join Date: Jan 2015 Posts: 25	Opentrons Automated Pipetting System Has anyone here used the Opentrons OT-2 for library preparation? https://opentrons.com/ot-2

06-15-2018, 03:10 PM	#2
Bukowski Senior Member Location: UK Join Date: Jan 2010 Posts: 390	"Accuracy Accurate down to 384-well plates" This is a new measure of accuracy that I was previously unaware of. Their only 'Sequencing Application' in their protocol library is for 16S. There are liquid handling platforms with a much wider array of NGS protocols ready to go than this..

06-15-2018, 06:09 PM	#3
luc Senior Member Location: US Join Date: Dec 2010 Posts: 429	A neighboring lab has the OT-One. This first version is unusable for just about anything - it can't hold the pipettes properly and the plates have a lot of play. Version two looks very different, though.

06-18-2018, 06:45 AM	#4
r.rosati Member Location: Brazil Join Date: Aug 2015 Posts: 92	There have recently been a few discussions about them on Reddit; perhaps it might be a way to get in touch with people who use them.

03-13-2019, 06:38 PM	#5
luc Senior Member Location: US Join Date: Dec 2010 Posts: 429	We recently got an OT2. So far it works for single channel pipetting. We have problems with both of our multi-channel pipettes. The magnetic module seems to have a design flaw - the magnet is too close to the samples, even when disengaged and at maximum distance (achieved with an self-made adapter). Thus, the beads do not stay in suspension for long - ideally they should stay in suspension for 5 minutes before then engaging the magnet.