Hi all.
I am new to the ChIP-seq and trying to ChIP genome-wide H3K27me3. Is there any methods or strategies for increasing the library complexity? Maybe shearing more using sonicator is one, but I am worrying about more sonication will hurt the K27 methylation and lead to poor IP. Or should I use more starting materials? Any thoughts would be grateful!
Wang
I am new to the ChIP-seq and trying to ChIP genome-wide H3K27me3. Is there any methods or strategies for increasing the library complexity? Maybe shearing more using sonicator is one, but I am worrying about more sonication will hurt the K27 methylation and lead to poor IP. Or should I use more starting materials? Any thoughts would be grateful!
Wang
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